植物生理学报

《植物生理学报》 2010, 46(3): 236-240

水稻粒长基因GL3 的遗传分析和分子标记定位

**高虹, 王嘉宇, 姜树坤, 孙健, 徐正进*, 陈温福**

沈阳农业大学水稻研究所, 辽宁省北方粳稻育种重点实验室, 农业部作物生理生态遗传育种重点开放实验室, 沈阳110866

通信作者：徐正进；E-mail: xuzhengjin@126.com；Tel: 024-88487183

摘　要：

为了解析水稻粒长的遗传机制, 以大粒水稻品种 ‘80018-TR161-2-1’ 和小粒水稻品种 ‘ 日本小黑稻 ’ 及其 F2 代 200 个株系和 F2:3 家系为材料, 分析水稻粒长的遗传学性状。结果表明, 谷粒长度的分离比在 F2 及 F2:3 家系中都表现为 3:1, 长粒性状受1对隐性核基因控制, 命名为GL3。用简单重复序列(simple sequence repeat, SSR)分子标记结合群体分组混合分析的方法, 将此种基因定位在水稻第 3 号染色体上 SSR 标记 PSM379 和 RM16 之间, 它们的遗传距离分别为 4.0 cM 和 11.2 cM。

关键词：水稻; 粒长; 遗传分析; 基因定位

收稿：2009-12-17 修定：2010-01-15

资助：辽宁省科技攻关项目(2006201002)。

Genetic Analysis and Molecular Mapping of the Gene GL3 for Grain Length in Rice (Oryza sativa L. var. japonica)

GAO Hong, WANG Jia-Yu, JIANG Shu-Kun, SUN Jian, XU Zheng-Jin*, CHEN Wen-Fu

Key Laboratory of Crop Physiology, Ecology, Genetics and Breeding, Ministry of Agriculture, Key Laboratory of Northern Japonica Rice Breeding of Liaoning Province, Rice Institute, Shenyang Agricultural University, Shenyang 110866, China

Corresponding author: XU Zheng-Jin; E-mail: xuzhengjin@126.com; Tel: 024-88487183

Abstract:

Genetic mechanism for grain length trait in rice (Oryza sativa) was analyzed by using F₂ population and F_2:3 family derived from the parents of large grain rice ‘80018-TR161-2-1’and small grain rice ‘small black rice of Japan’. The results showed the distribution of both grain length in F_{2 population and F_2:3 family expressed the separation ratio of 3:1. The long grain trait was controlled by a single recessive nuclear gene, named GL3. By using simple sequence repeat consorting with population group mixed analysis, the GL3 gene was mapped between two SSR markers PSM379 and RM16 on chromosome 3, whose genetic distances were 4.0 cM and 11.2 cM, respectively.
Key words: Oryza sativa; grain length; genetic analysis; gene location

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